国产传媒国产福利9|亚洲www啪成人一区二区麻豆国产av一区二区凹凸精品|九色91欧美亚洲精品一区二三区|国产真实交换配乱吟91日韩高清带中文字幕|欧美精品中文字幕亚洲专区久久久精品国产自拍|国产在线观看91精品腿张开|糖心vlog免费版下载|国产精品久久久久久久久久99|色成人av|伊人中文字幕在线观看,糖心vlog是什么,久久精品国产亚洲av高潮,国产青草91精品免费

Individual lenti microRNA inhibitors are designed using a proprietary algorithm, which is based on the work of Haraguchi, T, et al. and in collaboration with Dr. Hideo Iba, University of Tokyo. This algorithm utilizes the tough decoy (TuD) design. miRNA are known to regulate gene expression in a variety of manners, including translational repression, mRNA cleavage and deadenylation. The lentiviral microRNA Inhibitors are cloned into the TRC2-pLKO-puro vector. Co-transfection of this vector into the appropriate cell line with compatible packaging plasmids produces viral particles that can be used to transduce mammalian cells. Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory Element2 (WPRE) is included, allowing for enhanced expression of transgenes delivered by lentiviral vectors. This lentiviral vector also carries a puromycin resistance gene for selection of cells.

• Allows for potent inhibition of the desired miRNA
• Lentiviral delivery format allows for efficient delivery of the inhibitor into a wide variety of cell types
• Enables long-term inhibition without repeat transfection