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Phospho-FAK (Tyr576/577) Antibody

商城價：登錄可見

品牌：CST/賽信通

貨號：3281T

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商品名稱	規(guī)格	代理級別	價格	運(yùn)費(fèi)	供應(yīng)商	購買
CST 3281T Phospho-FAK(Tyr576/577) Antibody 交貨周期:部分現(xiàn)貨，期貨3-4周左右，優(yōu)質(zhì)售后	20μl	經(jīng)銷	登錄可見	-	上海優(yōu)寧維生物科技股份有限公司	- + 立即購買庫存：999

產(chǎn)品詳情

文獻(xiàn)引用

文獻(xiàn)圖片

實驗數(shù)據(jù)

應(yīng)用：W, IP
反應(yīng)性:C, X
Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration, and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for the Src family kinases PI3K and PLCgamma (2-5). Recruitment of Src family kinases results in the phosphorylation of Tyr407, Tyr576, and Tyr577 in the catalytic domain, and Tyr871 and Tyr925 in the carboxy-terminal region of FAK (6,7).Tyr576 and Tyr577 lie in the activation loop of the kinase domain, and mutation of these residues reduces FAK catalytic activity (6).

Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	125
SOURCE	Rabbit

Application Key:

W-Western
IP-Immunoprecipitation
IHC-Immunohistochemistry
ChIP-Chromatin Immunoprecipitation
IF-Immunofluorescence
F-Flow Cytometry
E-P-ELISA-Peptide

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Hm-Hamster
Mk-Monkey
Vir-Virus
Mi-Mink
C-Chicken
Dm-D. melanogaster
X-Xenopus
Z-Zebrafish
B-Bovine
Dg-Dog
Pg-Pig
Sc-S. cerevisiae
Ce-C. elegans
Hr-Horse
All-All Species Expected

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-FAK (Tyr576/577) Antibody detects endogenous levels of FAK only when phosphorylated at tyrosine 576/577. This antibody may cross-react with other activated receptor tyrosine kinases.

Species Reactivity:

Human, Mouse, Rat

Species predicted to react based on 100% sequence homology:

Chicken, Xenopus

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr576/577 of human FAK. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration, and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for the Src family kinases PI3K and PLCγ (2-5). Recruitment of Src family kinases results in the phosphorylation of Tyr407, Tyr576, and Tyr577 in the catalytic domain, and Tyr871 and Tyr925 in the carboxy-terminal region of FAK (6,7).

Tyr576 and Tyr577 lie in the activation loop of the kinase domain, and mutation of these residues reduces FAK catalytic activity (6).

Parsons, J.T. et al. (2000) Oncogene 19, 5606-13.
Schaller, M.D. et al. (1994) Mol Cell Biol 14, 1680-8.
Cobb, B.S. et al. (1994) Mol Cell Biol 14, 147-55.
Chen, H.C. et al. (1996) J Biol Chem 271, 26329-34.
Zhang, X. et al. (1999) Proc Natl Acad Sci U S A 96, 9021-6.
Calalb, M.B. et al. (1995) Mol Cell Biol 15, 954-63.
Schlaepfer, D.D. et al. (1994) Nature 372, 786-791.