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Phospho-Stat5 (Tyr694) (D47E7) XP Rabbit mAb

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品牌：CST/賽信通

貨號：4322T

規(guī) 格：

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DeepBio得分是基于文獻引用次數(shù)，影響因子，文獻新近度等因素計算的客觀產品評級，得分越高表明該產品經過越可靠的實驗驗證，質量可信度越高

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商品名稱	規(guī)格	代理級別	價格	運費	供應商	購買
CST 4322T Phospho-Stat5(Tyr694)(D47E7)XP Rabbit mAb 交貨周期:部分現(xiàn)貨，期貨3-4周左右，優(yōu)質售后	20μl	經銷	登錄可見	-	上海優(yōu)寧維生物科技股份有限公司	- + 立即購買庫存：997

產品詳情

文獻引用

文獻圖片

實驗數(shù)據(jù)

應用：W, IF-IC, F
反應性:R, B
Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6).

Supporting Data

REACTIVITY	H M
SENSITIVITY	Endogenous
MW (kDa)	90
Source/Isotype	Rabbit IgG

Application Key:

W-Western
IP-Immunoprecipitation
IHC-Immunohistochemistry
ChIP-Chromatin Immunoprecipitation
IF-Immunofluorescence
F-Flow Cytometry
E-P-ELISA-Peptide

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Hm-Hamster
Mk-Monkey
Vir-Virus
Mi-Mink
C-Chicken
Dm-D. melanogaster
X-Xenopus
Z-Zebrafish
B-Bovine
Dg-Dog
Pg-Pig
Sc-S. cerevisiae
Ce-C. elegans
Hr-Horse
All-All Species Expected

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunofluorescence (Immunocytochemistry)	1:100
Flow Cytometry	1:100 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-Stat5 (Tyr694) (D47E7) XP? Rabbit mAb detects endogenous levels of Stat5a only when phosphorylated at Tyr694 and Stat5b when phosphorylated at Tyr699.

Species Reactivity:

Human, Mouse

Species predicted to react based on 100% sequence homology:

Rat, Bovine

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr694 of human Stat5a protein.

Background

Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6).

Gouilleux, F. et al. (1994) EMBO J 13, 4361-9.
Wakao, H. et al. (1994) EMBO J 13, 2182-91.
Okutani, Y. et al. (2001) Oncogene 20, 6643-50.
Demoulin, J.B. et al. (1999) J Biol Chem 274, 25855-61.
Dentelli, P. et al. (1999) J. Immunol. 163, 2151-2159.
Meinke, A. et al. (1996) Mol Cell Biol 16, 6937-44.