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Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody

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品牌：CST/賽信通

貨號：2701T

規(guī) 格：

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商品名稱	規(guī)格	代理級別	價格	運費	供應(yīng)商	購買
CST 2701T Phospho-Zap-70(Tyr319)/Syk(Tyr352) Antibody 交貨周期:部分現(xiàn)貨，期貨3-4周左右，優(yōu)質(zhì)售后	20μl	經(jīng)銷	登錄可見	-	上海優(yōu)寧維生物科技股份有限公司	- + 立即購買庫存：999

產(chǎn)品詳情

文獻(xiàn)引用

文獻(xiàn)圖片

實驗數(shù)據(jù)

應(yīng)用：W, IF-IC, F
反應(yīng)性:M, R, Hm, Mk, C, B, Dg, Pg, Hr
The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).Phosphorylation of Tyr319 is required for the assembly of a Zap-70-containing signaling complex that leads to the activation of the PLC-gamma1-dependent and Ras-dependent signaling cascades in antigen-stimulated T cells (5,6). The orthologous Tyr352 residue in Syk is also involved in the association with PLC-gamma1 (9).

Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	70 Zap-70, 72 Syk
SOURCE	Rabbit

Application Key:

W-Western
IP-Immunoprecipitation
IHC-Immunohistochemistry
ChIP-Chromatin Immunoprecipitation
IF-Immunofluorescence
F-Flow Cytometry
E-P-ELISA-Peptide

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Hm-Hamster
Mk-Monkey
Vir-Virus
Mi-Mink
C-Chicken
Dm-D. melanogaster
X-Xenopus
Z-Zebrafish
B-Bovine
Dg-Dog
Pg-Pig
Sc-S. cerevisiae
Ce-C. elegans
Hr-Horse
All-All Species Expected

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunofluorescence (Immunocytochemistry)	1:50
Flow Cytometry	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody detects endogenous levels of Zap-70 only when phosphorylated at Tyr319. It cross-reacts with endogenous levels of Syk when phosphorylated at Tyr352.

Species Reactivity:

Human

Species predicted to react based on 100% sequence homology:

Mouse, Rat, Hamster, Monkey, Chicken, Bovine, Dog, Pig, Horse

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr319 of human Zap-70. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).

Phosphorylation of Tyr319 is required for the assembly of a Zap-70-containing signaling complex that leads to the activation of the PLC-gamma1-dependent and Ras-dependent signaling cascades in antigen-stimulated T cells (5,6). The orthologous Tyr352 residue in Syk is also involved in the association with PLC-gamma1 (9).

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Neumeister, E.N. et al. (1995) Mol Cell Biol 15, 3171-8.
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Williams, B.L. et al. (1999) EMBO J 18, 1832-44.
Di Bartolo, V. et al. (1999) J Biol Chem 274, 6285-94.
Wiestner, A. et al. (2003) Blood 101, 4944-51.
Crespo, M. et al. (2003) N Engl J Med 348, 1764-75.
Law, C.L. et al. (1996) Mol Cell Biol 16, 1305-15.