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Alternative Name:CRTH2; PTGDR2 ; Prostaglandin D2 receptor 2; DL1R; DP2; GPR44｜Application:Flow cytometry (Routinely Tested)｜Brand:BD Pharmingen?｜Immunogen:Human CRTH2 Transfected Cell Line｜Isotype:Rat WI, also known as Wistar (outbred) IgG2a, κ｜Reactivity:Human (QC Testing)｜Regulatory Status:RUO｜RRID:AB_2112699｜Storage Buffer:Aqueous buffered solution containing ≤0.09% sodium azide.｜Workshop Number:VIII 80349｜描述： The BM16 monoclonal antibody specifically binds to CD294. CD294 is encoded by PTGDR2 (Prostaglandin D2 receptor 2) and is also known as CRTH2 (chemoattractant receptor-homologous molecule expressed on Th2 cells), GPR44 (G protein-coupled receptor 44), DL1R, and DP2. CD294 is a member of the G protein-coupled leukocyte chemoattractant receptor family. CD294 is expressed on Th2 cells and type-2 innate lymphoid cells (ILC2), but not Th1 type cells. CD294 is detectable on CD4+ T cells in fresh PBMC but not on B cells and NK cells. CD294 is also expressed on peripheral blood basophils and eosinophils, suggesting its involvement allergic reactions. Phenotypic analysis of CD4+ T cells expressing CRTH2 demonstrated that they were also CD45RA-negative and CD45RO+ and CD25+. These cells produce Th2- but little or no Th1-type cytokines upon stimulation with PMA and Ionomycin. 商品通知：Since applications vary, each investigator should titrate the reagent to obtain optimal results.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.For fluorochrome spectra and suitable instrument settings, An isotype control should be used at the same concentration as the antibody of interest.The Alexa Fluor?, Pacific Blue?, and Cascade Blue? dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor? dyes (except for Alexa Fluor? 430), Pacific Blue? dye, and Cascade Blue? dye are covered by pending and issued patents.Alexa Fluor? 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).Alexa Fluor? is a registered trademark of Molecular Probes, Inc., Eugene, OR.