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Alternative Name:ITGA6; IThe GoH3 monoclonal ntegrin alpha-6; Integrin α6 chain; VLA-6; ITA6｜Application:Flow cytometry (Routinely Tested)｜Brand:BD Pharmingen?｜Immunogen:Mouse mammary tumor cells｜Isotype:Rat SD, also known as Sprague-Dawley (outbred) IgG2a, κ｜Reactivity:Human (QC Testing), Mouse, Pig, Dog (Tested in Development)｜Regulatory Status:RUO｜RRID:AB_396078｜Storage Buffer:Aqueous buffered solution containing BSA and ≤0.09% sodium azide.｜Vol. Per Test:20 μl｜Workshop Number:IV P55｜描述： The GoH3 monoclonal antibody specifically binds to CD49f which is also known as integrin α6 chain. CD49f is a ~150 kDa type I transmembrane glycoprotein that belongs to the integrin alpha chain family of extracellular matrix and cell adhesion receptors. The integrin α6 subunit associates with the integrin β1 chain (CD29) to form VLA-6 and with the integrin β4 chain (CD104) to form the integrin α6β4 complex, also known as the laminin and kalinin receptor. CD49f is expressed mainly on T cells, monocytes, platelets, epithelial cells, endothelial cells, perineural cells, and trophoblasts of placenta. GoH3 recognizes an extracellular epitope of integrin α6 on human, mouse and bovine cells. GoH3 has been reported to block the binding of integrin α6 to laminin P1 and E8 fragments. ｜商品通知：This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental sample (a test).An isotype control should be used at the same concentration as the antibody of interest.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.Source of all serum proteins is from USDA inspected abattoirs located in the United States.Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at .｜推薦的實驗流程：BD? CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.