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Alternative Name:IFNG; Interferon-gamma; Interferon-γ; Type II interferon; MAF｜Application:Intracellular staining (flow cytometry) (Routinely Tested)｜Brand:BD Pharmingen?｜Immunogen:Human IFN-γ Recombinant Protein｜Isotype:Mouse IgG1, κ｜Reactivity:Rhesus, Cynomolgus, Baboon (QC Testing), Human (Tested in Development)｜Regulatory Status:RUO｜RRID:AB_394516｜Storage Buffer:Aqueous buffered solution containing BSA and ≤0.09% sodium azide.｜Vol. Per Test:20 μl｜描述： The B27 monoclonal antibody specifically binds to human interferon-γ (IFN-γ), a 14-18 kDa glycoprotein containing 143 amino acid residues. IFN-γ is a potent multifunctional cytokine produced by several activated cell types including NK, NKT, CD4+TCRαβ+, CD8+TCRαβ+, and TCRγδ+ T cells. IFN-γ exerts its biological effects through specific binding to the high-affinity IFN-γ receptor complex comprised of IFN-γRα (CD119) and IFN-γRβ subunits. In addition to its antiviral effects, IFN-γ upregulates a number of lymphoid cell functions including the antimicrobial and anti-tumor responses of macrophages, NK cells, and neutrophils. In addition, IFN-γ influences the regulation of proliferation, differentiation, and effector responses of B cell and T cell subsets. These influences can involve IFN-γ's capacity to boost MHC class I and II expression by antigen-presenting cells as well as direct effects on B cells and T cells themselves. B27 is a neutralizing antibody. The use of B27 antibody for epitope mapping of human IFN-γ has been described. The B27 antibody has been reported not to bind to denatured IFN-γ. ｜商品通知：This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental sample (a test).An isotype control should be used at the same concentration as the antibody of interest.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.Source of all serum proteins is from USDA inspected abattoirs located in the United States.For fluorochrome spectra and suitable instrument settings, Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.