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Alternative Name:Integrin α4 chain; Integrin alpha 4; ITGA4; IA4; alpha 4 subunit of VLA-4｜Application:Flow cytometry (Routinely Tested)｜Brand:BD Pharmingen?｜Isotype:Mouse IgG1, κ｜Reactivity:Human (QC Testing), Rhesus, Cynomolgus, Baboon, Dog, Cow, Sheep, Cat, Horse (Tested in Development)｜Regulatory Status:RUO｜RRID:AB_395893｜Storage Buffer:Aqueous buffered solution containing BSA and ≤0.09% sodium azide.｜Vol. Per Test:20 μl｜Workshop Number:V S215｜描述： The 9F10 monoclonal antibody specifically reacts with the integrin α4 chain, that is expressed as a heterodimer with either of two β integrin subunits, β1 (CD29) or β7. The α4β1 integrin (VLA-4) is expressed on lymphocytes, monocytes, thymocytes, NK cells, and several B- and T-cell lines, and mediates binding to VCAM-1 (CD106) and the CS-1 region of fibronectin. The α4β7 integrin has a similar tissue distribution, except it is found on only a small subpopulation of thymocytes. Integrin α4β7 also binds fibronectin and VCAM-1, and has been shown in the mouse to preferentially bind the mucosal vascular addressin molecule, MAdCAM-1. This antibody is useful for studies of the expression by and function of cells that express α4 chain-containing integrins. This clone cross-reacts with a subset of peripheral blood lymphocytes, monocytes, and some granulocytes of baboon and both rhesus and cynomolgus macaque monkeys. The distribution on leukocytes is similar to that observed with human peripheral blood leukocytes. ｜商品通知：This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental sample (a test).Since applications vary, each investigator should titrate the reagent to obtain optimal results.For fluorochrome spectra and suitable instrument settings, Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.Source of all serum proteins is from USDA inspected abattoirs located in the United States.