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Inhibit proliferation of cancer cells by inhibiting topoisomerase I activity with camptothecin. Highly potent and cell-permeable, camptothecin inhibits topoisomerase I from religating broken DNA strands by forming a complex with topoisomerase I and DNA, which leads to cell death (Thomas et al.). Camptothecin has been used for: CANCER RESEARCH · Can be used as an apoptosis induction positive control (cell-type dependent) in combination with Caspase-3/7 Activity Flow Cytometry Kit, Green (Catalog #100-0923), Caspase-3/7 Activity Plate Reader Assay Kits (Catalog #100-0922/100-0920), and FITC-C6-DEVD-FMK (Catalog #100-0924). · Demonstrates cytotoxicity against several human tumor cell lines, including HT29, LOX, SKOV3, and SKVLB (Hentze et al.). · Promotes degradation of mixed lineage leukemia 5 (MLL5) proteins in HCT116 cells (Cheng et al.). · Induces apoptosis in various human cancer cell lines (HeLa, K562, CCRF-CEM, HCT-15, RBE, PC3, and C-33A) via a microRNA-125b-mediated mitochondrial pathway (Zeng et al.).