MCE 的所有產(chǎn)品僅用作科學研究或藥證申報,，我們不為任何個人用途提供產(chǎn)品和服務,。
CAS No. : 25316-40-9
MCE 國際站：Doxorubicin (hydrochloride) (Standard)
產(chǎn)品活性：Doxorubicin hydrochloride (Standard)是 Doxorubicin hydrochloride 的分析標準品。本產(chǎn)品用于研究及分析應用,。Doxorubicin (Hydroxydaunorubicin) hydrochloride 是一種具有細胞毒性的蒽環(huán)類抗生素,，是一種抗癌化療試劑。Doxorubicin hydrochloride 是一種有效的人類 DNA topoisomerase I 和 topoisomerase II 抑制劑,，IC50 分別為 0.8 μM 和 2.67 μM,。Doxorubicin hydrochloride 可降低 AMPK 及其下游靶蛋白乙酰輔酶 A 羧化酶的磷酸化。還可誘導凋亡 (apoptosis) 和自噬,。
研究領域：Cell Cycle/DNA Damage | Antibody-drug Conjugate/ADC Related | PI3K/Akt/mTOR | Epigenetics | Autophagy | Apoptosis | Anti-infection
作用靶點：Topoisomerase | ADC Cytotoxin | AMPK | Autophagy | Apoptosis | HIV | HBV | Mitophagy | Antibiotic | Bacterial
In Vitro: Doxorubicin (1-8 μM; 24 and 48 hours) hydrochloride decreases the viability of MCF-10F, MCF-7 and MDA-MB-231 cells in a time- and dose-dependent manner.
Doxorubicin (1 μM; 3 and 24 hours) hydrochloride results in Hct-116 human colon carcinoma cells reduction in G0/G1 phase and accumulation in G2 phase.
Doxorubicin (1 μM for MCF-10F and MDA-MB-231 cells, 4 μM for MCF-7 cells; 48 hours) hydrochloride induces apoptosis by upregulating Bax, caspase-8 and caspase-3 and downregulation of Bcl-2 protein expression.
Doxorubicin (5 μM; 10-30 min) hydrochloride can be accumulated in B16-F10 melanoma cell line CRL-6475 in a time-dependent manner, and can be detected by green or red fluorescence (green fluorescence has higher detection sensitivity) with a maximum excitation wavelength (λex) and a maximum emission wavelength (λem) of 470 nm and 560 nm, respectively.
Note:
Doxorubicin hydrochloride is suitable for neutral or weakly acidic solvents; PBS (PH 7.4) is not recommended for dissolution, which may affect the dissolution effect.
In Vivo: Doxorubicin hydrochloride can be used to induce models of cardiotoxicity and heart failure.
1. Inducing cardiotoxicity
Background
The mechanism of short-term induction of cardiotoxicity: promoting extracellular remodeling of myocardium to induce heart failure, and also works at the molecular level. Including: interacting with iron, changing the activity of oxidases in cells or mitochondria, and binding to topoisomerases.
Specific Mmodeling Methods
Mice: Female C57BL/6j mice ? 20-22 g ? 6 weeks old
Administration: 2 mg/kg, 10 mg/kg ? ip ? once every the other day for 2 or 3 times
Note
Mice were sacrificed 3 days after the first injection.
Modeling Indicators
molecular level: (1) Pro-inflammatory cytokines (such as TNF-α and IL-6) increase significantly, while anti-inflammatory cytokine IL-10 decreases; (2) Inducible - Nitric oxide synthase (iNOS) was overexpressed and serum nitrite levels were elevated; nitrotyrosine expression was significantly increased.
Correlated Product(s): /
Opposite Product(s): /
2. Induction of heart failure
Background
The long-term mechanisms inducing heart failure remain to be carefully elucidated.
Specific Mmodeling Methods
Rat: Male Wistar rats ? 180-200 g
Administration: 2.5 mg/kg ? ip ? once every week, for 6 weeks ? treated at 4 weeks later.
Modeling Indicators
Cellular/tissue level: Myocardial sarcomeres are destroyed, mitochondria are swollen and damaged,
Myocardial cells showed inflammation and apoptosis (transmission electron microscopy results); myocardial fibrosis, and left ventricular collagen I and II levels were downregulated (immunohistochemistry results).
Changes in macroscopic indicators: Hemodynamic parameters and echocardiography show cardiac insufficiency and impaired left ventricular function; such as increases in LVIDd, LVIDs and LVEDP.
Correlated Product(s): /
Opposite Product(s): Captopril (HY-B0368)
相關(guān)產(chǎn)品：MG-132 | Rapamycin | Dimethyl sulfoxide, meets analytical specification of Ch.P. | Cycloheximide | Chloroquine | 3-Methyladenine | Tamoxifen | Doxorubicin hydrochloride | Bafilomycin A1 | Dexamethasone | Y-27632 | 2-Deoxy-D-glucose | Paclitaxel | Olaparib | Angiotensin II human | Z-VAD-FMK | Acetylcysteine | LY294002 | Actinomycin D | SB-431542 | Staurosporine | 5-Fluorouracil | Puromycin dihydrochloride | Oxaliplatin | Bortezomib | Sorafenib | Trametinib | Dorsomorphin | Etoposide | Gemcitabine
熱門產(chǎn)品線：重組蛋白 | 藥物篩選 | 天然產(chǎn)物 | 熒光染料 | PROTAC | 同位素標記物
Trending products：Recombinant Proteins | Bioactive Screening Libraries | Natural Products | Dye Reagents | PROTAC | Isotope-Labeled Compounds
品牌介紹:
? MCE (MedChemExpress) 擁有200 多種全球獨家化合物庫,，我們致力于為全球科研客戶提供前沿最全的高品質(zhì)小分子活性化合物；
? 50,000 多種高選擇性抑制劑,、激動劑涉及各熱門信號通路及疾病領域,；
? 產(chǎn)品種類涵蓋各種重組蛋白，多肽，常用試劑盒 ,，更有 PROTAC,、ADC 等特色產(chǎn)品，廣泛應用于新藥研發(fā),、生命科學等科研項目,；
? 提供虛擬篩選，離子通道篩選,，代謝組學分析檢測分析,，藥物篩選等專業(yè)技術(shù)服務；
? 設有專業(yè)的實驗中心和嚴格的質(zhì)控,、驗證體系,；
? 提供 LC/MS、NMR,、HPLC,、手性分析、元素分析等各項質(zhì)檢報告,，確保產(chǎn)品的高純度,、高品質(zhì)；
? 產(chǎn)品的生物活性多經(jīng)各國客戶實驗驗證,；
? Nature, Cell, Science 等多種頂級期刊及制藥專利收錄了MCE客戶的科研成果,；
? 專業(yè)團隊跟蹤最新的制藥及生命科學研究進展，為您提供全球最新的活性化合物,；
? 與世界各大制藥公司及知名科研機構(gòu)建立了長期的合作,。
分類糾錯
舉報
糾錯
收 藏
比價
算.png?x-oss-process=image/resize,h_600,w_600){kind=small}
線下結(jié)算
公務卡銀支付.png?x-oss-process=image/resize,h_600,w_600){kind=small}
網(wǎng)銀支付
微信
支付寶
在線客服
店內(nèi)搜索
