MCE 的所有產(chǎn)品僅用作科學(xué)研究或藥證申報,，我們不為任何個人用途提供產(chǎn)品和服務(wù)。

DiR

CAS No. : 100068-60-8

MCE 國際站：DiR

產(chǎn)品活性：DiR 是一種長鏈碳菁染料,。碳菁染料廣泛用作親脂性示蹤劑,，用于標(biāo)記細胞、細胞器,、脂質(zhì)體,、病毒和脂蛋白。

研究領(lǐng)域：Others

作用靶點：Fluorescent Dye

In Vitro: Carbocyanine dyes are widely used as Di to label cells, organelles, liposomes, viruses and lipoproteins. Long-chain carbocyanines which include DiO (DiOC18(3)), DiI (DiIC18(3)), DiD (DiIC18(5)) and DiR, and dialkyl aminostyryl dye DiA (4-Di-16-ASP) are used for labeling membranes and other hydrophobic structures. DiIC16(3) has shorter alkyl substituents (C16) than DiI (C18). They have extremely high extinction coefficients, environmental dependent fluorescence and short excited-state lifetimes in lipid environments. They are oils at room temperature and weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes or bound to lipophilic biomolecules. These optical characteristics make them ideal for staining the cytoplasmic membranes of cells. Once applied to cells, these dyes diffuse laterally within the plasma membrane, resulting in staining of the entire cell.
DiO, DiI, DiD and DiR exhibit distinct green, orange, red and infrared fluorescence, respectively thus facilitating multicolor imaging and flow cytometric analysis of live cells . DiO and DiI can be used with standard FITC and TRITC filters respectively. Among them DiI and its analogs are most frequently used since they usually exhibit very low cell toxicity. In addition, DiI is widely used for determining lipoproteins such as LDL and HDL. The lipophilic aminostyryl dye DiA is also often used for neuronal tracing.
General Protocol
1. Preparing Stain Solutions of Di
a. Prepare DMF, DMSO or ethanol stock solutions: The stock solutions should be prepared in dimethyl formamide (DMF), dimethylsulfoxide (DMSO, or ethanol DMSO at 1-5 mM. DMF is preferable to ethanol as a solvent for Di. The stock solution should be used promptly. Any unused solution need to be aliquoted and refrozen at least -20℃. Avoid repeated freeze/thaw cycle. The solution can be stored for 6 months.
b. Prepare working solutions: Dilute the stock solutions into a suitable buffer such as serum-free culture medium, HBSS or PBS to make 1 to 5 μM working solutions. We do not recommend storing the aqueous solution for more than one day.
Note: The final concentration of the working solution should be empirically determined for different cell types and/or experimental conditions.
2. Suspension cells
a. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×10⁶/mL.
b. Add 1 mL of Di working solution, and then incubate at room temperature for 5-30 minutes.
c. Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend cells with serum-free cell culture medium or PBS.Observation by fluorescence microscopy or flow cytometry.
3. Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells, and then incubate at room temperature for 5-30 minutes.
d. Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry.

相關(guān)產(chǎn)品：MitoSOX Red  |  BODIPY 493/503  |  D-Luciferin potassium  |  Nile Red  |  FITC  |  Propidium Iodide  |  JC-1  |  Dihydroethidium  |  2-NBDG  |  CFDA-SE  |  FITC-Dextran (MW 4000)  |  DiI  |  Cy5.5  |  PKH 26  |  Fluo-4 AM  |  CY5  |  BODIPY 581/591 C11  |  DiD perchlorate  |  TMRE  |  Calcein-AM  |  RhoNox-1  |  Rhod-2 AM  |  Thiazolyl Blue  |  DiO  |  Indocyanine green  |  Biotinyl tyramide  |  MitoTracker Green FM

熱門產(chǎn)品線：重組蛋白  |  藥物篩選  |  天然產(chǎn)物  |  熒光染料  |  PROTAC  |  同位素標(biāo)記物

Trending products：Recombinant Proteins  |  Bioactive Screening Libraries  |  Natural Products  |  Dye Reagents  |  PROTAC  |  Isotope-Labeled Compounds

品牌介紹:
?   MCE (MedChemExpress) 擁有200 多種全球獨家化合物庫,，我們致力于為全球科研客戶提供前沿最全的高品質(zhì)小分子活性化合物,；
?   50,000 多種高選擇性抑制劑、激動劑涉及各熱門信號通路及疾病領(lǐng)域,；
?   產(chǎn)品種類涵蓋各種重組蛋白,，多肽，常用試劑盒 ,，更有 PROTAC,、ADC 等特色產(chǎn)品,，廣泛應(yīng)用于新藥研發(fā)、生命科學(xué)等科研項目,；
?   提供虛擬篩選,，離子通道篩選，代謝組學(xué)分析檢測分析,，藥物篩選等專業(yè)技術(shù)服務(wù),；
?   設(shè)有專業(yè)的實驗中心和嚴格的質(zhì)控、驗證體系,；
?   提供 LC/MS,、NMR、HPLC,、手性分析,、元素分析等各項質(zhì)檢報告，確保產(chǎn)品的高純度,、高品質(zhì),；
?   產(chǎn)品的生物活性多經(jīng)各國客戶實驗驗證；
?   Nature, Cell, Science 等多種頂級期刊及制藥專利收錄了MCE客戶的科研成果,；
?   專業(yè)團隊跟蹤最新的制藥及生命科學(xué)研究進展,，為您提供全球最新的活性化合物；
?   與世界各大制藥公司及知名科研機構(gòu)建立了長期的合作,。