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JC-1

CAS No. : 3520-43-2

MCE 國際站：JC-1

產(chǎn)品活性：JC-1 (CBIC2) 是一種廣泛用于檢測線粒體膜電位的理想熒光探針,。JC-1 染料以電勢依賴性的方式積聚在線粒體內(nèi),，可以用來檢測細(xì)胞、組織或純化的線粒體膜電位,。正常線粒體內(nèi),，JC-1 聚集在線粒體基質(zhì)中形成聚合物，聚合物發(fā)出強烈的紅色熒光 (Ex=488 nm, Em=595 nm) ,；在線粒體膜電位較低時,，JC-1不能聚集在線粒體的基質(zhì)中，產(chǎn)生綠色熒光 (Ex=488 nm, Em=530 nm) ,。

研究領(lǐng)域：Others

作用靶點：Fluorescent Dye

In Vitro: JC-1 staining
a. Take the 6-well plate as an example for cell planking, and the density is 5×10⁵/mL. Incubate overnight in 5% CO₂ incubator at 37℃.
Note: it is suggested that the cell density during apoptosis induction should not exceed 1×10⁶/ml, which can also be cultured to the appropriate density according to your own cell type.
b. Take 0.5 mL suspension into sterile centrifuge tube; 400 g centrifugation for 3-5 min; Discard the supernatant.
c. The cells were resuspended with 1mljc-1 working solution and incubated in 5% CO₂ incubator at 37℃for 15-30 min.
d. Centrifugation at room temperature for 5 min at 400 g; Suck of the supernatant.
e. The cells were resuspended with 2 mL cell culture medium or buffer, and then centrifuged at room temperature for 5 min at 400 g; Discard the supernatant and repeat twice.
f. Resuspend the cells with 1mL of fresh culture medium or buffer, and immediately conduct subsequent flow cytometry or fluorescence microscope observation.
g. Data analysis (flow cytometry) : mitochondria of healthy cells containing red JC-1 aggregates were detected by FL2 channel; Apoptotic or unhealthy cells containing green JC-1 monomer were detected by FL1 (FITC) channel.
h. If used for enzyme labeling instrument, use 300 μL buffer resuspended cells; Then 100 per hole μ Transfer the stained cells to a light tight 96 well plate with the amount of L, and then conduct fluorescent enzyme label plate analysis.

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