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CAS No. : 273221-67-3
MCE 國際站:Fluo-4 AM
產(chǎn)品活性:Fluo-4 AM 是一種常用的檢測細胞內(nèi) Ca2+ (鈣離子) 濃度的探針,。
研究領域:Others
作用靶點:Fluorescent Dye
In Vitro: Fluo-4 AM is a fluorescent dye (λex=494 nm, λem=516 nm). Preloaded with Fuo-4 AM, a very bright fluorescence image is observed. In a parallel experiment with fluo-3 AM-loaded cells, the resulting fluorescence image, although clearly discernable in this case, is less bright.
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Count the cells and take 106 cells from each sample (control and experiment/s).
2. Collect the cells (5 min, 3000×g, 4 °C) and wash once in PBS.
3. Resuspend the cells in 0.5-ml PBS and add 0.5 μl of Fluo-4-AM (1 mM stock) to a final concentration of 1 μM. Incubate at 37 °C for 1 h.
4. Wash the cells three times (2 min, 3000×g) with PBS and finally resuspend in 1-ml PBS. Separate into 2 flow cytometry tubes—0.5 ml in each.
5. Evaluate the staining by flow cytometry and analyze the data by a software such as CellQuest software.
相關產(chǎn)品:MitoSOX Red | BODIPY 493/503 | D-Luciferin potassium | Nile Red | FITC | DiR | Propidium Iodide | JC-1 | Dihydroethidium | 2-NBDG | CFDA-SE | FITC-Dextran (MW 4000) | DiI | Cy5.5 | PKH 26 | CY5 | BODIPY 581/591 C11 | DiD perchlorate | TMRE | Calcein-AM | RhoNox-1 | Rhod-2 AM | Thiazolyl Blue | DiO | Indocyanine green | Biotinyl tyramide | MitoTracker Green FM
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Trending products:Recombinant Proteins | Bioactive Screening Libraries | Natural Products | Dye Reagents | PROTAC | Isotope-Labeled Compounds
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