MCE 的所有產(chǎn)品僅用作科學(xué)研究或藥證申報,，我們不為任何個人用途提供產(chǎn)品和服務(wù)。

Propidium Iodide

CAS No. : 25535-16-4

MCE 國際站：Propidium Iodide

產(chǎn)品活性：Propidium Iodide (PI) 是一種細(xì)胞核染色試劑,，可染色 DNA,。Propidium Iodide 是一種溴化乙啶的類似物，在嵌入雙鏈 DNA 后釋放紅色熒光,。Propidium Iodide 不能通過活細(xì)胞膜,，但卻能穿過破損的細(xì)胞膜而對核染色。Propidium Iodide 的熒光波長為 493/617 nm,與 DNA 嵌合后波長為 536/635 nm,。Propidium Iodide 常用于細(xì)胞凋亡 (apoptosis) 或細(xì)胞壞死 (necrosis) 的檢測,，常用于流式細(xì)胞儀分析。

研究領(lǐng)域：Others  |  Cell Cycle/DNA Damage

作用靶點：Fluorescent Dye  |  DNA/RNA Synthesis

In Vitro: "General Protocol

1.Preparation of PI working solution
1.1 Preparation of the stock solution
Dissolve 1 mg PI in 1 mL DDH₂O to obtain 1 mg/mL of stock solution.
Note: It is recommended to store the stock solution at -20°C or -80°C away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of PI working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 20-50 μg/mL of working solution.
Note: Please adjust the concentration of PI working solution according to the actual situation.
2.Cell staining
2.1 Suspension cells（6-well plate）
a.Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL.
b.Add 1 mL of working solution, and then incubate at room temperature for 5-10 minutes.
c.Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
d.Wash twice with PBS, 5 minutes each time.
e.Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-10 minutes.
d. Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry.

Storage
-20°C, 1 year
Protect from light

Precautions
1. Please adjust the concentration of PI working solution according to the actual situation.
2. This product is for R&D use only, not for drug, household, or other uses.
3. For your safety and health, please wear a lab coat and disposable gloves to operate.
"

相關(guān)產(chǎn)品：Cycloheximide  |  MitoSOX Red  |  Actinomycin D  |  Oxaliplatin  |  BODIPY 493/503  |  Gemcitabine  |  D-Luciferin potassium  |  Bleomycin sulfate  |  Mitomycin C  |  Carboplatin  |  Streptozotocin  |  Cytarabine  |  Nile Red  |  FITC  |  DiR  |  5-BrdU  |  Methotrexate  |  JC-1  |  Anisomycin  |  Dihydroethidium  |  2-NBDG  |  Fludarabine  |  CFDA-SE  |  Thymidine  |  FITC-Dextran (MW 4000)  |  DiI  |  ART558  |  Cy5.5  |  Hydroxyurea

熱門產(chǎn)品線：重組蛋白  |  藥物篩選  |  天然產(chǎn)物  |  熒光染料  |  PROTAC  |  同位素標(biāo)記物

Trending products：Recombinant Proteins  |  Bioactive Screening Libraries  |  Natural Products  |  Dye Reagents  |  PROTAC  |  Isotope-Labeled Compounds

品牌介紹:
?   MCE (MedChemExpress) 擁有200 多種全球獨家化合物庫,，我們致力于為全球科研客戶提供前沿最全的高品質(zhì)小分子活性化合物,；
?   50,000 多種高選擇性抑制劑、激動劑涉及各熱門信號通路及疾病領(lǐng)域,；
?   產(chǎn)品種類涵蓋各種重組蛋白,，多肽，常用試劑盒 ,，更有 PROTAC,、ADC 等特色產(chǎn)品，廣泛應(yīng)用于新藥研發(fā),、生命科學(xué)等科研項目,；
?   提供虛擬篩選，離子通道篩選,，代謝組學(xué)分析檢測分析,，藥物篩選等專業(yè)技術(shù)服務(wù)；
?   設(shè)有專業(yè)的實驗中心和嚴(yán)格的質(zhì)控,、驗證體系,；
?   提供 LC/MS、NMR,、HPLC,、手性分析、元素分析等各項質(zhì)檢報告,，確保產(chǎn)品的高純度,、高品質(zhì),；
?   產(chǎn)品的生物活性多經(jīng)各國客戶實驗驗證；
?   Nature, Cell, Science 等多種頂級期刊及制藥專利收錄了MCE客戶的科研成果,；
?   專業(yè)團隊跟蹤最新的制藥及生命科學(xué)研究進(jìn)展,，為您提供全球最新的活性化合物；
?   與世界各大制藥公司及知名科研機構(gòu)建立了長期的合作,。