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BODIPY 493/503

CAS No. : 121207-31-6

MCE 國(guó)際站：BODIPY 493/503

產(chǎn)品活性：BODIPY493/503 是一種 BODIPY 染料。BODIPY 染料是一種具有強(qiáng)紫外吸收能力的小分子染料,，其熒光峰比較尖銳,，量子產(chǎn)率高。其對(duì)環(huán)境的極性和 pH 值相對(duì)不敏感,，因此,，在不同生理?xiàng)l件下相對(duì)穩(wěn)定。由于其結(jié)構(gòu)的非對(duì)稱性,，BODIPY 具有多種衍生結(jié)構(gòu)產(chǎn)物,。BODIPY 脂滴類染料可以很好的穿過(guò)細(xì)胞膜進(jìn)入細(xì)胞內(nèi)部，通過(guò)在細(xì)胞內(nèi)的中性脂類上定位以進(jìn)行特異性染色,，因此,，該染料可用于標(biāo)記活細(xì)胞和固定細(xì)胞。最大激發(fā)/發(fā)射波長(zhǎng)：493/503 nm,。

研究領(lǐng)域：Others

作用靶點(diǎn)：Fluorescent Dye

In Vitro: General Protocol
1. Preparation of BODIPY 493/503 working solution
1.1 Preparation of the stock solution
Dissolve 1 mg BODIPY 493/503 in 382 μL DMSO to obtain 10 mM of stock solution.
Note: It is recommended to store the stock solution at -20°C or -80°C, keep away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of BODIPY 493/503 working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution.
Note: Please adjust the concentration of BODIPY 493/503 working solution according to the actual situation.
2. Cell staining
2.1 Suspension cells (6-well plate)
a. Centrifuge at 1000 g at 4°C for 3-5 min and then discard the supernatant. Wash twice with PBS, 5 min each time.The cell density is 1×10⁶/mL.
b. Add 1 mL of working solution, and then incubate at room temperature for 5-30 min.
c.Centrifuge at 400 g at 4°C for 3-4 min and then discard the supernatant.
d. Wash twice with PBS, 5 min each time.
e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 min.
d. Wash twice with medium, 5 min each time. Observation by fluorescence microscopy or flow cytometry.

Storage
-20°C, 1 year; Protect from light.
Precautions
1. Please adjust the concentration of BODIPY 493/503 working solution according to the actual situation.
2. Experiments suggest a positive control, incubate the control group cells with 30 μM oleic acid for 8 h and then perform subsequent experiments.
3. This product is only for R&D use, not for drug, household, or others.
4. For your safety and health, please wear a lab coat and disposable gloves to operate.

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