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Alternative Name:1G8 Antigen; Esam; Esam1; endothelial cell-selective adhesion molecule｜Application:Flow cytometry (Qualified)｜Brand:BD OptiBuild?｜Concentration:0.2 mg/ml｜Immunogen:Mouse endothelial Cell Line｜Isotype:Rat LEW, also known as Lewis IgG2a, κ｜Reactivity:Mouse (Tested in Development)｜Regulatory Status:RUO｜Storage Buffer:Aqueous buffered solution containing ≤0.09% sodium azide.｜描述： The 1G8 monoclonal antibody specifically recognizes Endothelial cell-selective adhesion molecule (ESAM). ESAM is a ~55 kDa single-pass type I transmembrane glycoprotein that is encoded by ESAM (Endothelial cell-specific adhesion molecule) which belongs to the immunoglobulin supergene superfamily (IgSF). ESAM contains an IgV-type and IgC2-type domain in its extracellular region followed by a transmembrane sequence and cytoplasmic tail. ESAM is strongly expressed on platelets, megakaryocytes, and endothelial cells at interendothelial cell junctions as well as dendritic cells. Through homophilic interactions, this interendothelial cell adhesion molecule may play roles in regulating vascular permeability and in the extravasation of leucocytes, eg, neutrophils, through blood vessel walls. ESAM is also reportedly expressed on hematopoietic stem cells (HSCs). ESAM-positive cell populations are enriched for multipotent myeloid erythroid progenitors and primitive progenitors with lymphopoietic activity. ｜商品通知：Researchers should determine the optimal concentration of this reagent for their individual applications.The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.An isotype control should be used at the same concentration as the antibody of interest.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.CF? is a trademark of Biotium, Inc.For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page Since applications vary, each investigator should titrate the reagent to obtain optimal results.｜推薦的實(shí)驗(yàn)流程： BD? CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD? CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD? CompBeads to ensure that BD? CompBeads are appropriate for your specific cellular application.