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Alternative Name:NCAM1; NCAM-1; NCAM; Leu-19; Neural cell adhesion molecule 1; NKH1; MSK39｜Application:Flow cytometry (Qualified)｜Brand:BD OptiBuild?｜Concentration:0.2 mg/ml｜Entrez Gene ID:4684｜Immunogen:Immunoaffinity-enriched adult human brain NCAM｜Isotype:Mouse BALB/c IgG2b, κ｜Reactivity:Human (Tested in Development)｜Regulatory Status:RUO｜Storage Buffer:Aqueous buffered solution containing ≤0.09% sodium azide.｜Workshop Number:V NK60｜描述： The NCAM16.2 monoclonal antibody specifically binds to human CD56. It recognizes an extracellular immunoglobulin-like domain common to 120, 140, and 180 kDa forms of CD56, also known as the neural cell adhesion molecule (NCAM), NKH1 or MSK39. The CD56 antigen is expressed on approximately 10% to 25% of peripheral blood lymphocytes. It is present on essentially all resting and activated CD16+ natural killer (NK) lymphocytes and approximately 5% of CD3+ peripheral blood lymphocytes. CD3+ CD56+ T lymphocytes comprise a unique subset of cytotoxic T lymphocytes that mediates non-major histocompatibility complex (MHC)-restricted cytotoxicity. CD56 antigen density on NK lymphocytes increases upon cellular activation. The CD56 antigen is involved in neuronal homotypic cell adhesion and cell differentiation during embryogenesis. CD16+ CD56+ NK cells demonstrate reciprocal transfer of an activation state with dendritic cells. ｜商品通知：Researchers should determine the optimal concentration of this reagent for their individual applications.The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.An isotype control should be used at the same concentration as the antibody of interest.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.CF? is a trademark of Biotium, Inc.For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page Since applications vary, each investigator should titrate the reagent to obtain optimal results.Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant? Stain Buffer in your experiments to help mitigate potential background. ｜推薦的實(shí)驗(yàn)流程： BD? CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD? CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD? CompBeads to ensure that BD? CompBeads are appropriate for your specific cellular application.