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Helios (D8W4X) XP Rabbit mAb

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品牌：CST/賽信通

貨號：42427T

規(guī) 格：

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DeepBio得分:

DeepBio得分是基于文獻引用次數(shù)，影響因子，文獻新近度等因素計算的客觀產(chǎn)品評級，得分越高表明該產(chǎn)品經(jīng)過越可靠的實驗驗證，質(zhì)量可信度越高

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商品名稱	規(guī)格	代理級別	價格	運費	供應(yīng)商	購買
Helios (D8W4X) XP ? Rabbit mAb 交貨周期:現(xiàn)貨	20 μl	經(jīng)銷	登錄可見	-	北京敏泰元科技有限公司	- + 立即購買庫存：20
CST 42427T Helios(D8W4X)XP Rabbit mAb 交貨周期:部分現(xiàn)貨，期貨3-4周左右，優(yōu)質(zhì)售后	20μl	經(jīng)銷	登錄可見	-	上海優(yōu)寧維生物科技股份有限公司	- + 立即購買庫存：999

產(chǎn)品詳情

文獻引用

文獻圖片

實驗數(shù)據(jù)

應(yīng)用：W, IP, F, ChIP, ChIP-seq
Helios (Ikaros family zinc finger 2, IZKF2) is an Ikaros family transcription factor composed of several zinc fingers that mediate DNA binding and homodimerization or heterodimerization with other Ikaros family proteins (1,2). In the hematopoietic system, Helios expression is restricted to T cells and early hematopoietic progenitors (1,2). In regulatory T cells, expression of Helios contributes to an anergic phenotype by binding to the IL-2 promoter and suppressing IL-2 transcription (3). In addition, alteration of the corresponding Helios gene IZKF2 is one hallmark of low-hypodiploid acute lymphoblastic leukemia (4).

Supporting Data

REACTIVITY	H M
SENSITIVITY	Endogenous
MW (kDa)	58, 63-70, 85
Source/Isotype	Rabbit IgG

Application Key:

W-Western
IP-Immunoprecipitation
IHC-Immunohistochemistry
ChIP-Chromatin Immunoprecipitation
IF-Immunofluorescence
F-Flow Cytometry
E-P-ELISA-Peptide

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Hm-Hamster
Mk-Monkey
Vir-Virus
Mi-Mink
C-Chicken
Dm-D. melanogaster
X-Xenopus
Z-Zebrafish
B-Bovine
Dg-Dog
Pg-Pig
Sc-S. cerevisiae
Ce-C. elegans
Hr-Horse
All-All Species Expected

Product Usage Information

For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10⁶ cells) per IP. This antibody has been validated using SimpleChIP^?Enzymatic Chromatin IP Kits.

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:200
Flow Cytometry	1:100
Chromatin IP	1:50
Chromatin IP-seq	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Helios (D8W4X) XP^? Rabbit mAb recognizes endogenous levels of total Helios protein.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly73 of human Helios protein.

Background

Helios (Ikaros family zinc finger 2, IZKF2) is an Ikaros family transcription factor composed of several zinc fingers that mediate DNA binding and homodimerization or heterodimerization with other Ikaros family proteins (1,2). In the hematopoietic system, Helios expression is restricted to T cells and early hematopoietic progenitors (1,2). In regulatory T cells, expression of Helios contributes to an anergic phenotype by binding to the IL-2 promoter and suppressing IL-2 transcription (3). In addition, alteration of the corresponding Helios gene IZKF2 is one hallmark of low-hypodiploid acute lymphoblastic leukemia (4).

Hahm, K. et al. (1998) Genes Dev 12, 782-96.
Kelley, C.M. et al. (1998) Curr Biol 8, 508-15.
Baine, I. et al. (2013) J Immunol 190, 1008-16.
Holmfeldt, L. et al. (2013) Nat Genet 45, 242-52.