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LC3A/B (D3U4C) XP Rabbit mAb

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品牌：CST/賽信通

貨號(hào)：12741T

規(guī) 格：

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DeepBio得分:

DeepBio得分是基于文獻(xiàn)引用次數(shù)，影響因子，文獻(xiàn)新近度等因素計(jì)算的客觀產(chǎn)品評(píng)級(jí)，得分越高表明該產(chǎn)品經(jīng)過(guò)越可靠的實(shí)驗(yàn)驗(yàn)證，質(zhì)量可信度越高

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商品名稱	規(guī)格	代理級(jí)別	價(jià)格	運(yùn)費(fèi)	供應(yīng)商	購(gòu)買(mǎi)
LC3A/B (D3U4C) XP ? Rabbit mAb 交貨周期:現(xiàn)貨	20 μl	經(jīng)銷(xiāo)	登錄可見(jiàn)	-	北京敏泰元科技有限公司	- + 立即購(gòu)買(mǎi) 庫(kù)存：20
CST 12741T LC3A/B(D3U4C)XP Rabbit mAb 交貨周期:部分現(xiàn)貨，期貨3-4周左右，優(yōu)質(zhì)售后	20μl	經(jīng)銷(xiāo)	登錄可見(jiàn)	-	上海優(yōu)寧維生物科技股份有限公司	- + 立即購(gòu)買(mǎi) 庫(kù)存：991

產(chǎn)品詳情

文獻(xiàn)引用

文獻(xiàn)圖片

實(shí)驗(yàn)數(shù)據(jù)

應(yīng)用：W, IHC-P, IF-IC, F
反應(yīng)性:X, B, Dg, Pg
Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation, but it has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection, and cancer (3). Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4) and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy (5). Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo posttranslational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10). The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form, LC3-II, have been used as indicators of autophagy (11).

Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	14, 16
Source/Isotype	Rabbit IgG

Application Key:

W-Western
IP-Immunoprecipitation
IHC-Immunohistochemistry
ChIP-Chromatin Immunoprecipitation
IF-Immunofluorescence
F-Flow Cytometry
E-P-ELISA-Peptide

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Hm-Hamster
Mk-Monkey
Vir-Virus
Mi-Mink
C-Chicken
Dm-D. melanogaster
X-Xenopus
Z-Zebrafish
B-Bovine
Dg-Dog
Pg-Pig
Sc-S. cerevisiae
Ce-C. elegans
Hr-Horse
All-All Species Expected

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunohistochemistry (Paraffin)	1:500
Immunofluorescence (Immunocytochemistry)	1:100
Flow Cytometry	1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

LC3A/B (D3U4C) XP^? Rabbit mAb recognizes endogenous levels of total LC3A and LC3B proteins.

Species Reactivity:

Human, Mouse, Rat

Species predicted to react based on 100% sequence homology:

Xenopus, Bovine, Dog, Pig

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu44 of human LC3B protein (conserved in LC3A).

Background

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation, but it has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection, and cancer (3). Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4) and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy (5). Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo posttranslational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10). The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form, LC3-II, have been used as indicators of autophagy (11).

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