喀斯瑪商城-第三方B2B電子商務(wù)平臺

Cox2 (D5H5) XP Rabbit mAb

商城價：登錄可見

品牌：CST/賽信通

貨號：12282T

規(guī) 格：

運費：登錄后可查看運費

DeepBio得分:

DeepBio得分是基于文獻引用次數(shù)，影響因子，文獻新近度等因素計算的客觀產(chǎn)品評級，得分越高表明該產(chǎn)品經(jīng)過越可靠的實驗驗證，質(zhì)量可信度越高

1家供應(yīng)商在售
商品名稱	規(guī)格	代理級別	價格	運費	供應(yīng)商	購買
Cox2 (D5H5) XP ? Rabbit mAb 交貨周期:現(xiàn)貨	20 μl	經(jīng)銷	登錄可見	-	北京敏泰元科技有限公司	- + 立即購買庫存：19

產(chǎn)品詳情

文獻引用

文獻圖片

實驗數(shù)據(jù)

應(yīng)用：W, IHC-P, IF-IC, F
Cyclooxygenase1 (Cox1) and cyclooxygenase2 (Cox2), family members with 60% homology in humans, catalyze prostaglandin production from arachidonic acid (1,2). While Cox1 expression is constitutive in most tissues, Cox2 expression is induced by lipopolysaccharide (LPS) and peptidoglycan (PGN) (3). PGN activates Ras, leading to phosphorylation of Raf at Ser338 and Erk1/2 at Tyr204. The activation of MAP kinase signaling results in subsequent activation of IKKalpha/beta, phosphorylation of IkappaBalpha at Ser32/36, and NF-kappaB activation. Finally, activation of the transcription factor NF-kappaB is responsible for the induction of Cox2 expression (4). Investigators have shown that LPS and PGN induce the clinical manifestations of arthritis and bacterial infections, such as inflammation, fever, and septic shock (5). Research studies have indicated that Cox1 and Cox2 may also play a role in the neuropathology of Alzheimer's disease by potentiating gamma-secretase activity and beta-amyloid generation (6).

Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	74
Source/Isotype	Rabbit IgG

Application Key:

W-Western
IP-Immunoprecipitation
IHC-Immunohistochemistry
ChIP-Chromatin Immunoprecipitation
IF-Immunofluorescence
F-Flow Cytometry
E-P-ELISA-Peptide

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Hm-Hamster
Mk-Monkey
Vir-Virus
Mi-Mink
C-Chicken
Dm-D. melanogaster
X-Xenopus
Z-Zebrafish
B-Bovine
Dg-Dog
Pg-Pig
Sc-S. cerevisiae
Ce-C. elegans
Hr-Horse
All-All Species Expected

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunohistochemistry (Paraffin)	1:600
Immunofluorescence (Immunocytochemistry)	1:800
Flow Cytometry	1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Cox2 (D5H5) XP^? Rabbit mAb recognizes endogenous levels of total Cox2 protein. Non-specific labeling of centrioles may be observed by immunofluorescence in human cells.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His108 of human Cox2 protein.

Background

Cyclooxygenase1 (Cox1) and cyclooxygenase2 (Cox2), family members with 60% homology in humans, catalyze prostaglandin production from arachidonic acid (1,2). While Cox1 expression is constitutive in most tissues, Cox2 expression is induced by lipopolysaccharide (LPS) and peptidoglycan (PGN) (3). PGN activates Ras, leading to phosphorylation of Raf at Ser338 and Erk1/2 at Tyr204. The activation of MAP kinase signaling results in subsequent activation of IKKα/β, phosphorylation of IκBα at Ser32/36, and NF-κB activation. Finally, activation of the transcription factor NF-κB is responsible for the induction of Cox2 expression (4). Investigators have shown that LPS and PGN induce the clinical manifestations of arthritis and bacterial infections, such as inflammation, fever, and septic shock (5). Research studies have indicated that Cox1 and Cox2 may also play a role in the neuropathology of Alzheimer's disease by potentiating γ-secretase activity and β-amyloid generation (6).

Xie, W.L. et al. (1991) Proc Natl Acad Sci USA 88, 2692-6.
Vane, J.R. et al. (1998) Annu Rev Pharmacol Toxicol 38, 97-120.
O'Neill, G.P. et al. (1994) Mol Pharmacol 45, 245-54.
Chen, B.C. et al. (2004) J Biol Chem 279, 20889-97.
Wang, Q. et al. (2001) Infect Immun 69, 2270-6.
Qin, W. et al. (2003) J Biol Chem 278, 50970-7.
Onodera, Y. et al. (2015) FEBS Open Bio 5, 492-501.